Expression of 20β-hydroxysteroid dehydrogenase and P450 17α-hydroxylase/c17-20 lyase during hCG-induced in vitro oocyte maturation in snake head murrel Channa striatus

dc.contributor.author Sreenivasulu, G.
dc.contributor.author Swapna, I.
dc.contributor.author Rasheeda, M. K.
dc.contributor.author Ijiri, S.
dc.contributor.author Adachi, S.
dc.contributor.author Thangaraj, K.
dc.contributor.author Senthilkumaran, B.
dc.date.accessioned 2022-03-27T00:58:33Z
dc.date.available 2022-03-27T00:58:33Z
dc.date.issued 2005-04-01
dc.description.abstract Partial cDNAs encoding carbonyl reductase like 20β-hydroxysteroid dehydrogenase (20β-HSD) and P450 17α-hydroxylase/c17-20 lyase (CYP17) were isolated from the ovary of snake head murrel and they exhibited high sequence identity to the Nile tilapia and rainbow trout, respectively. A low transcript level of both 20β-HSD and CYP17 were detected in pre-vitellogenic follicles, while the transcript level was high in full-grown immature follicles. In hCG-induced in vitro oocyte maturation, we found a significant increase in 20β-HSD transcript level after 2 h. The CYP17 transcripts also showed a considerable increase following hCG-induction compared to saline-treated controls. On the other hand, Western blot analysis demonstrated no significant change in the CYP17 protein level during hCG-induced in vitro oocyte maturation. Taken together, we suggest that in addition to 20β-HSD, the CYP17 might have a role in the shift in steroidogenesis during meiotic maturation of snake head murrel. © Springer Science+Business Media B.V. 2006.
dc.identifier.citation Fish Physiology and Biochemistry. v.31(2-3)
dc.identifier.issn 09201742
dc.identifier.uri 10.1007/s10695-006-0028-z
dc.identifier.uri http://link.springer.com/10.1007/s10695-006-0028-z
dc.identifier.uri https://dspace.uohyd.ac.in/handle/1/3611
dc.subject 20β-HSD
dc.subject Channa striatus
dc.subject CYP17
dc.subject Oocyte maturation
dc.subject Steroidogenic shift
dc.title Expression of 20β-hydroxysteroid dehydrogenase and P450 17α-hydroxylase/c17-20 lyase during hCG-induced in vitro oocyte maturation in snake head murrel Channa striatus
dc.type Journal. Conference Paper
dspace.entity.type
Files
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Plain Text
Description: