Insights into the carbonic anhydrases and autotrophic carbon dioxide fixation pathways of high CO < inf > 2 < /inf > tolerant Rhodovulum viride JA756

dc.contributor.author Khandavalli, Lakshmi Venkata Naga Satya
dc.contributor.author Lodha, Tushar
dc.contributor.author Abdullah, Maaged
dc.contributor.author Guruprasad, Lalitha
dc.contributor.author Chintalapati, Sasikala
dc.contributor.author Chintalapati, Venkata Ramana
dc.date.accessioned 2022-03-27T08:33:52Z
dc.date.available 2022-03-27T08:33:52Z
dc.date.issued 2018-10-01
dc.description.abstract Biofixation of CO2 is being extensively investigated to solve the global warming problem. Purple non-sulfur bacteria are fast growers that consume CO2 and produce beneficial biomass. Better the growth at higher CO2 levels, more efficient are the strains for biofixation. Nine among fifty strains that were analyzed at elevated CO2 levels responded with better growth. Considering its enhanced growth at high CO2 and metabolic versatility, Rhodovulum viride strain JA756 was chosen to make further studies. Strain JA756 tolerates up to 50% (v/v) CO2 with its optimum between 20–40% (v/v), yielding a biomass of 3.4 g. L−1. The pattern of specific enzyme activity of carbonic anhydrase corresponded well with that of its growth. To gain insights into the genomic composition and genes related to carbonic anhydrases and CO2 fixation, draft genome sequencing of JA756 was carried out which revealed the presence of two non-homologous genes encoding for β and γ carbonic anhydrases, both of which are assumed to be implicated in maintaining intracellular inorganic carbon concentration at equilibrium. Most of the genes involved in the Calvin pathway, reductive tricarboxylic acid pathway, 3-hydroxypropionate bicycle and C4 pathways were found in the draft genome. While the experimental determinations of active roles of two of these pathways are still underway, the expression of key genes of Calvin and C4 pathway suggest their functional role in the organism. Owing to its metabolic versatility, JA756 can be advantageous for biological CO2 assimilation facilities located by the coastline, inland and also at wide ranges of CO2 concentrations.
dc.identifier.citation Microbiological Research. v.215
dc.identifier.issn 09445013
dc.identifier.uri 10.1016/j.micres.2018.07.006
dc.identifier.uri https://www.sciencedirect.com/science/article/abs/pii/S0944501318301757
dc.identifier.uri https://dspace.uohyd.ac.in/handle/1/10813
dc.subject Autotrophic
dc.subject Biofixation
dc.subject Carbonic anhydrase
dc.subject CO fixation pathway 2
dc.subject High CO cells 2
dc.subject Rhodovulum viride JA756
dc.title Insights into the carbonic anhydrases and autotrophic carbon dioxide fixation pathways of high CO < inf > 2 < /inf > tolerant Rhodovulum viride JA756
dc.type Journal. Article
dspace.entity.type
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