Inhibition of 5-lipoxygenase by vitamin E

dc.contributor.author	Reddanna, Pallu
dc.contributor.author	Krishna Rao, M.
dc.contributor.author	Channa Reddy, C.
dc.date.accessioned	2022-03-27T00:57:31Z
dc.date.available	2022-03-27T00:57:31Z
dc.date.issued	1985-11-25
dc.description.abstract	Purified 5-lipoxygenase from potato tubers was inhibited strongly by vitamin E and its analogs. The inhibition by d-α-tocopherol was found to be irreversible and non-competitive with respect to arachidonic acid. An IC50 of 5μM was calculated for d-α-tocopherol. The inhibition appears to be unrelated to its antioxidant function. Binding studies with 14C-labelled d-α-tocopherol revealed that there is a strong interaction between vitamin E and 5-lipoxygenase. Tryptic digestion and peptide mapping of 5-lipoxygenase-vitamin E complex indicate that vitamin E binds strongly to a single peptide. These studies suggest that cellular vitamin E levels may have profound influence on the formation of leukotrienes. © 1985.
dc.identifier.citation	FEBS Letters. v.193(1)
dc.identifier.issn	00145793
dc.identifier.uri	10.1016/0014-5793(85)80075-2
dc.identifier.uri	http://doi.wiley.com/10.1016/0014-5793%2885%2980075-2
dc.identifier.uri	https://dspace.uohyd.ac.in/handle/1/3434
dc.subject	5-Lipoxygenase
dc.subject	Antioxidant
dc.subject	Enzyme inhibition
dc.subject	Leukotriene
dc.subject	Vitamin E
dc.subject	α-Tocopherol
dc.title	Inhibition of 5-lipoxygenase by vitamin E
dc.type	Journal. Article
dspace.entity.type

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