Malonate-inhibition of allosteric phosphoenolpyruvate carboxylase from Setaria italica

Abstract

Malonate inhibited phosphoenolpyruvate carboxylase activity in crude extracts of Setaria italica Beauv. and Pennisetum typhoides S & H leaves. Kinetic studies revealed the competetive inhibition of partially purified phosphoenolpyruvate carboxylase preparation from Setaria italica. Glucose-6-phosphate activated the enzyme. Oxalacetate also competetively inhibited the enzyme and malate in a noncompetetive manner. Results clearly indicated the allosteric nature of phosphoenolpyruvate carboxylase from Setaria italica. © 1975 Academic Press, Inc.