Biochemistry - Publicatons

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Browsing Biochemistry - Publicatons by Subject "3DM"
  • Item
    Protein Engineering of α/β-Hydrolase Fold Enzymes
    ( 2011-07-01) Jochens, Helge ; Hesseler, Martin ; Stiba, Konstanze ; Padhi, Santosh Kumar ; Kazlauskas, Romas J. ; Bornscheuer, Uwe T.
    The superfamily of α/β-hydrolase fold enzymes is one of the largest known protein families, including a broad range of synthetically useful enzymes such as lipases, esterases, amidases, hydroxynitrile lyases, epoxide hydrolases and dehalogenases. This minireview covers methods developed for efficient protein engineering of these enzymes. Special emphasis is placed on the alteration of enzyme properties such as substrate range, thermostability and enantioselectivity for their application in biocatalysis. In addition, concepts for the investigation of the evolutionary relationship between the different members of this protein superfamily are covered, together with successful examples. Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
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    The α/β-hydrolase fold 3DM database (ABHDB) as a tool for protein engineering
    ( 2010-08-16) Kourist, Robert ; Jochens, Helge ; Bartsch, Sebastian ; Kuipers, Remko ; Padhi, Santosh Kumar ; Gall, Markus ; Böttcher, Dominique ; Joosten, Henk Jan ; Bornscheuer, Uwe T.
    (Figure Presented) Aligning the haystack to expose the needle: The 3DM method was used to generate a comprehensive database of the α/β- hydrolase fold enzyme superfamily. This database facilitates the analysis of structure-function relationships and enables novel insights into this superfamily to be made. In addition high-quality libraries for protein engineering can be easily designed. © 2010 Wiley-VCH Verlag GmbH & Co. KGaA.