Department of Plant Sciences

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Browsing Department of Plant Sciences by Subject "2DE"
  • Item
    Accumulation of transcription factors and cell signaling-related proteins in the nucleus during citrus-Xanthomonas interaction
    ( 2015-07-01) Rani, T. Swaroopa ; Durgeshwar, P. ; Podile, Appa Rao
    The nucleus is the maestro of the cell and is involved in the modulation of cell signaling during stress. We performed a comprehensive nuclear proteome analysis of Citrus sinensis during interaction with host (Xanthomonas citri pv. citri-Xcc) and non-host (Xanthomonas oryzae pv. oryzae-Xoo) pathogens. The nuclear proteome was obtained using a sequential method of organelle enrichment and determined by nano-LC-MS/MS analysis. A total of 243 proteins accumulated differentially during citrus-. Xanthomonas interaction, belonging to 11 functional groups, with signaling and transcription-related proteins dominating. MADS-box transcription factors, DEAD-box RNA helicase and leucine aminopeptidase, mainly involved in jasmonic acid (JA) responses, were in high abundance during non-host interaction (Xoo). Signaling-related proteins like serine/threonine kinase, histones (H3.2, H2A), phosphoglycerate kinase, dynamin, actin and aldolase showed increased accumulation early during Xoo interaction. Our results suggest that there is a possible involvement of JA-triggered defense responses during non-host resistance, with early recognition of the non-host pathogen.
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    Proteins Associated with Oxidative Burst and Cell Wall Strengthening Accumulate During Citrus-Xanthomonas Non-Host Interaction
    ( 2015-10-31) Rani, T. Swaroopa ; Takahashi, Daisuke ; Uemura, Matsuo ; Podile, Appa Rao
    Citrus proteome changes at 8 and 48 h post inoculation (hpi) were analysed by both 2D gel electrophoresis and nano-LC-MS/MS proteomic approaches during interaction with Xanthomonas axonopodis pv. citri (Xac) and Xanthomonas oryzae pv. oryzae (Xoo) as host and non-host pathogens, respectively. A total of 256 proteins, 72 at 8 hpi and 184 at 48 hpi, differentially accumulated during citrus-Xanthomonas interaction. Of these, 67 and 115 proteins were specific to Xac and Xoo interaction, respectively. In addition, 64 proteins, 10 at 8 hpi and 54 at 48 hpi, variedly accumulated during both the interactions. Proteins related to photosynthesis, carbohydrate metabolism and protein synthesis were in low abundance during both the interactions resulting in reduced rate of photosynthesis. Proteins related to defence response, cell wall (CW) strengthening, lignin deposition and generation of reactive oxygen species (ROS) were in high abundance only during Xoo interaction. Whereas, during Xac interaction, proteins involved in antioxidant metabolism and CW loosening and/or elongation were in high abundance. The precise increase in abundance of these proteins during non-host interaction suggested an important role for CW fortification and ROS accumulation in non-host resistance in plants.