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Members of Gammaproteobacteria and Bacilli represent the culturable diversity of chitinolytic bacteria in chitin-enriched soils

( 2010-09-24) Das, Subha Narayan ; Sarma, P. V.S.R.N. ; Neeraja, Chilukoti ; Malati, Nampally ; Podile, Appa Rao

Culturable chitinolytic bacterial diversity was studied in chitin-rich soils collected from two industries involved in chitin production. A total of 27 chitinolytic isolates were isolated among which only 10 showed zone of clearance ≥4 mm on colloidal chitin agar plate. Using morphological, biochemical and 16S rDNA analysis, isolates were identified as Bacillus, Paenibacillus, Stenotrophomonas and Pseudomonas. Molecular phylogenetic analysis revealed that Gammaproteobacteria and Bacilli were found to be the predominant classes in these chitinenriched soils. Chitinolytic bacterial population densities were significantly high and showed a rather simple community composition dominated by genus Bacillus and Stenotrophomonas (74%). This is the first report on assessing the chitinolytic bacterial diversity of soils from industries involved in chitin production. © 2010 Springer Science+Business Media B.V.